Pesquisa | SciELO

Abstract In addition to destroying the leaves, stems, pods, and grains of soybean (Glycine max), Spodoptera frugiperda larvae may also have the cut off behavior in the seedlings close to the ground, harming the establishment of the soybean crop. Therefore, this study aimed to evaluate the consequences of the fall armyworm attack by adopting the cut off behavior, which has been similarly documented for black cutworm (Agrotis ipsilon) in newly emerged soybean plants. The treatments were five levels of infestation with 0, 5, 10, 20 and 40 larvae per m2. Three variables were assessed: (1) stand (relationship between attacked and initial number of plants), (2) types and amounts of injured structures, such as hypocotyl (embryonic shoot), and the cotyledons (seed leaves), and (3) level of defoliation. The variables were collected every 24 hours until the pupal stage. Additionally, the final stand of the seedlings was quantified, with the highest injury intensity observed in the plots that contained the highest number of larvae. In the second experiment, the dynamics of movement and attacks of plants by S. frugiperda larvae at the following times of the day: 07:00, 10:00, 13:00, 16:00, 19:00 and 21:00 were analyzed with supervised machine learning models. The injury caused by S. frugiperda with the behavior of the cut off and the voracity of the larvae were evident in the structures (hypocotyl and cotyledons) of the evaluated plants. The results of the present study emphasize the need to manage S. frugiperda during the preplanting phase of soybean.

https://doi.org/10.1590/1678-4324-2023220386

Emotional, hyperactivity and inattention problems in adolescents with immunocompromising chronic diseases during the COVID-19 pandemic
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Campos, Reinan T.

; Lindoso, Livia

; Sousa, Renan A. de

; Helito, Alberto C.

; Ihara, Bianca P.

; Strabelli, Claudia A.A.

; Paradelas, Levi M.V.

; Carneiro, Beatriz O.L.

; Cardoso, Maria Paula R.

; Souza, Jean Paulo V. de

; Freire, Marianna R. de M.

; Astley, Camilla

; Laurentino, Moisés F.

; Buscatti, Izabel M.

; Kozu, Katia

; Aikawa, Nadia E.

; Sallum, Adriana M.E.

; Ferreira, Juliana CO.

; Simon, Juliana R.

; Viana, Vivianne S.L.

; Queiroz, Ligia B.

; Gualano, Bruno

; Roschel, Hamilton

; Pereira, Rosa Maria R.

; Toma, Ricardo K.

; Watanabe, Andréia

; Grangeiro, Patricia M.

; Casella, Caio B.

; Farhat, Sylvia C.

; Polanczyk, Guilherme V.

; Campos, Lucia Maria A.

; Silva, Clovis A.

Clinics

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2023, Volume 78 elocation 100167

Resumo: En | Texto: En | PDF: En

Abstract Objective: To assess factors associated with emotional changes and Hyperactivity/Inattention (HI) motivated by COVID-19 quarantine in adolescents with immunocompromising diseases. Methods: A cross-sectional study included 343 adolescents with immunocompromising diseases and 108 healthy adolescents. Online questionnaires were answered including socio-demographic data and self-rated healthcare routine during COVID-19 quarantine and validated surveys: Strengths and Difficulties Questionnaire (SDQ), Pittsburgh Sleep Quality Index (PSQI), Pediatric Quality of Life Inventory 4.0 (PedsQL4.0). Results: The frequencies of abnormal emotional SDQ scores from adolescents with chronic diseases were similar to those of healthy subjects (110/343 [32%] vs. 38/108 [35%], p = 0.548), as well as abnormal hyperactivity/inattention SDQ scores (79/343 [23%] vs. 29/108 [27%], p = 0.417). Logistic regression analysis of independent variables associated with abnormal emotional scores from adolescents with chronic diseases showed: female sex (Odds Ratio [OR = 3.76]; 95% Confidence Interval (95% CI) 2.00–7.05; p < 0.001), poor sleep quality (OR = 2.05; 95% CI 1.08–3.88; p = 0.028) and intrafamilial violence during pandemic (OR = 2.17; 95% CI 1.12–4.19; p = 0.021) as independently associated with abnormal emotional scores, whereas total PedsQL score was inversely associated with abnormal emotional scores (OR = 0.95; 95% CI 0.93–0.96; p < 0.0001). Logistic regression analysis associated with abnormal HI scores from patients evidenced that total PedsQL score (OR = 0.97; 95% CI 0.95–0.99; p = 0.010], changes in medical appointments during the pandemic (OR = 0.39; 95% CI 0.19–0.79; p = 0.021), and reliable COVID-19 information (OR = 0.35; 95% CI 0.16–0.77; p = 0.026) remained inversely associated with abnormal HI scores. Conclusion: The present study showed emotional and HI disturbances in adolescents with chronic immunosuppressive diseases during the COVID-19 pandemic. It reinforces the need to promptly implement a longitudinal program to protect the mental health of adolescents with and without chronic illnesses during future pandemics.

https://doi.org/10.1016/j.clinsp.2023.100167

Determination of Tensile Properties of Polyester - Industrial Waste Red Mud Composites Using Digital Image Correlation (DIC)
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Rodrigues, Marcelo

; Ribeiro, Maurício Maia

; Cardoso, Robson Luis Baleeiro

; Ferreira, Ana Gabriele da Paixão

; Cardoso, Silmara Mota

; Silva, Jaciléa Campos da

; Ramos, Roberto Paulo Barbosa

; Rodrigues, Jean da Silva

Materials Research

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2023, Volume 26 elocation e20220449

Resumo: En | Texto: En | PDF: En

The Digital Image Correlation (DIC) technique is an important method of evaluating material strain fields. Composite materials have inherently heterogeneous elastic properties, in the function of the different phases present in the composition, whereat the traditional techniques of deformation evaluation may not be sufficient to determine the mechanisms that eventually contribute to the failure of the material. The present work were evaluated, the tensile mechanical properties of polyester matrix composites loaded with an industrial residue of red mud, with a mass fraction of 20%. The properties were surveyed using the conventional technique of strain gauge and compared with the data obtained through DIC. The results showed that the DIC technique was accurate in monitoring the displacements and determining the average deformation of the tested specimens, in addition to providing ample deformation fields, for the evaluation of failure mechanisms throughout the sample request process.

https://doi.org/10.1590/1980-5373-mr-2022-0449

Evaluation of konjac noodle as a microsurgery training model: learning curve analysis
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AVELAR, TIAGO MARQUES

; LOVATO, RENAN MAXIMILIAN

; BARBOSA, THIAGO GOMES

; XANDER, PAULO ADOLFO WESSEL

; RODRIGUES, LEONARDO HENRIQUE DA SILVA

; CAMPOS, ADRIANA JOSE BRITO

; RIECHELMANN, RICARDO SALEMI

; FLORES, JUAN ANTONIO CASTRO

; AGUIAR, GUILHERME BRASILEIRO DE

; OLIVEIRA, JEAN GONÇALVES DE

; VEIGA, JOSÉ CARLOS ESTEVES

Revista do Colégio Brasileiro de Cirurgiões

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2023, Volume 50 elocation e20233528

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT Background: classical models of microsurgical anastomosis training are expensive and have ethical implications. Some alternatives join low cost and easiness to store. However, the translation of knowledge acquired by training in these methods into the traditional ones is not clear. This project aims to assess the feasibility of konjac noodles as a reliable microsurgery-training model. Methods: 10 neurosurgery residents performed an end-to-end anastomosis in a 2-3mm placenta artery. The anastomoses were evaluated quantitatively, recording time; and qualitatively, applying a validated score (Anastomosis Lapse Index - ALI) by three experienced neurosurgeons and verifying the presence of gross leakage through the infusion of fluorescein. Subsequently, they performed 10 non-consecutive sessions of anastomosis training in the konjac noodle. Eventually, a final anastomosis in the placenta model was performed and the same parameters were scored. Results: we observed a 17min reduction in the mean time to perform the anastomosis in the placenta model after the training in the konjac (p<0.05). There was a non-significant 20% reduction in gross leakage, but the training sessions were not able to consistently improve the ALI score. Conclusions: we demonstrate a reduction in anastomosis performing time in placental arteries after training sessions in the konjac noodle model, which can be regarded as a feasible low-cost method, particularly useful in centers with surgical microscopes only in the operation room.

ABSTRACT Introdução: modelos tradicionais de treinamento de anastomose microcirúrgica costumam ter custos elevados e implicações éticas de aquisição e manutenção, buscando-se alternativas que reúnam baixo custo e facilidade de armazenamento. Existem diferentes propostas, porém há poucas evidências de que conhecimentos adquiridos com o treinamento nessas plataformas se traduza em melhora na performance, quando estes são comparados a modelos consagrados. Este projeto objetiva avaliar a viabilidade do macarrão de konjac como modelo confiável de treinamento microcirúrgico. Métodos: 10 residentes de neurocirurgia realizaram uma anastomose término-terminal em artéria placentária humana de 2-3mm. As anastomoses foram avaliadas quantitativamente, registrando-se o tempo de confecção e qualitativamente, aplicando-se um escore validado (Anastomosis Lapse Index – ALI) por neurocirurgiões experientes e verificando-se a presença de vazamento grosseiro através da visualização no modo fluorescente injetando-se fluoresceína. Subsequentemente, realizaram 10 sessões de treinamento não consecutivos de anastomose términoterminal no modelo de konjac. Por fim, uma anastomose final foi realizada no modelo placentário e os mesmo parâmetros reavaliados. Resultados: observamos uma redução de 17 min no tempo médio de confecção da anastomose no modelo de placenta após os treinos no modelo do macarrão (p<0.05). Houve uma redução não significativa de 20% no vazamento grosseiro. As sessões de treino no macarrão não foram capazes de melhorar consistentemente o score ALI. Conclusão: o treinamento em modelo de macarrão konjac é capaz de reduzir o tempo para realização das anastomoses no modelo em placenta humana, mostrando-se alternativa viável de baixo custo e manutenção, útil em serviços que disponham de microscópio apenas no ambiente cirúrgico.

https://doi.org/10.1590/0100-6991e-20233528-en

Potential of a native araticum species as the rootstock of atemoya cultivars propagated by grafting methods
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Silva, Edvan Costa da

; Villa, Fabíola

; Silva, Daniel Fernandes da

; Possenti, Jean Carlo

; Silva, Adriana de Castro Correia da

; Masiero, Michel Anderson

Acta Scientiarum. Agronomy

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2023, Volume 45 elocation e60964

Resumo: En | Texto: En | PDF: En

ABSTRACT. Technical and secure information on the production of quality atemoya seedlings (Annona squamosa L. x Annona cherimola Mill.) are of paramount importance due to the growing interest in this crop. This study aimed to assess the formation of atemoya seedlings propagated by grafting methods and cultivars using araticum (Annona sylvatica) rootstocks. The experiment was carried out in a screened environment at the Western Paraná State University (Unioeste), Campus of Marechal Cândido Rondon, Paraná State, Brazil. Atemoya grafts (Thompson, African Pride, and Gefner) were collected from plants from the CATI seedling nursery, located in São Bento do Sapucaí, São Paulo State, Brazil. Grafting was performed using 1-year-old araticum rootstocks with 8-10 mm in diameter at a height of 15 cm from the ground and grafts measuring, on average, 8.5 cm in length, 10 mm in diameter, and three buds. The experimental design consisted of randomized blocks in a 3 × 3 factorial arrangement (three grafting methods: cleft graft, whip and tongue graft, and chip budding × three cultivars: African Pride, Thompson, and Gefner), with four replications and 10 seedlings per replication, totaling 40 seedlings per treatment. The percentage of graft success (%), percentage of sprouting (%), number of sprouts, number of leaves, and longest sprout length (cm) were assessed at 60 days after grafting. The cleft graft method is efficient for producing atemoya seedlings. The cultivar African Pride can be grafted onto rootstocks by the cleft graft and whip and tongue graft methods, as they promote a higher percentage of graft success. The Annona sylvatica rootstock has potential for the production of atemoya seedlings, but chip budding is not viable.

https://doi.org/10.4025/actasciagron.v45i1.60964

Contribution of public oral pathology services to the diagnosis of oral and oropharyngeal cancer in Brazil
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LOUREDO, Brendo Vinicius Rodrigues

; CURADO, Maria Paula

; PENAFORT, Paulo Victor Mendes

; DE ARRUDA, José Alcides Almeida

; ABREU, Lucas Guimarães

; MESQUITA, Ricardo Alves

; PINTO-JÚNIOR, Décio dos Santos

; ABRAHÃO, Aline Corrêa

; ANDRADE, Bruno Augusto Benevenuto de

; AGOSTINI, Michelle

; MORAES, Renata Mendonça

; ANBINDER, Ana Lia

; DOURADO, Pedro Henrique Silva

; SANTOS, Teresa Cristina Ribeiro Bartholomeu dos

; PIRES, Fábio Ramoa

; BORDIGNON, Natalia Cristina Trentin

; GONDAK, Rogério Oliveira

; DE OLIVEIRA, Marcia Gaiger

; CARRARD, Vinicius Coelho

; MARTINS, Manoela Domingues

; SOUSA-NETO, Sebastião Silvério

; ARANTES, Diego Antônio Costa

; MENDONÇA, Elismauro Francisco

; CIESLAK-SANCHES, Silvia Roberta

; ANTUNES, Daniella Moraes

; AMARAL-SILVA, Gleyson Kleber do

; MANIERI, Patricia Rubia

; RAMALHO, Luciana Maria Pedreira

; DOS SANTOS, Jean Nunes

; LEONEL, Augusto César Leal da Silva

; PEREZ, Danyel Elias da Cruz

; VERHEUL, Hannah Carmem Carlos Ribeiro Silva

; BARROSO, Keila Martha Amorim

; RODRIGUES, Flávia Luiza Santos

; GONZAGA, Amanda Katarinny Goes

; FERNANDES, Romana Renery

; DE SOUZA, Lélia Batista

; SOUZA, Lucas Lacerda de

; PONTES, Flávia Sirotheau Corrêa

; PONTES, Hélder Antônio Rebelo

; SILVA, Caroline Alfaia

; CÂMARA, Jeconias

; LIBÓRIO-KIMURA, Tatiana Nayara

; SANTOS-SILVA, Alan Roger

; LOPES, Márcio Ajudarte

; ALMEIDA, Oslei Paes de

; ROMAÑACH, Mário José

; VARGAS, Pablo Agustin

Brazilian Oral Research

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2023, Volume 37 elocation e126

Resumo: En | Texto: En | PDF: En

Abstract This study aimed to evaluate the contribution of oral and maxillofacial pathology laboratories (OMPLs) in Brazilian public universities to the diagnosis of lip, oral cavity, and oropharyngeal squamous cell carcinoma (SCC). A cross-sectional study was performed using biopsy records from a consortium of sixteen public OMPLs from all regions of Brazil (North, Northeast, Central-West, Southeast, and South). Clinical and demographic data of patients diagnosed with lip, oral cavity, and oropharyngeal SCC between 2010 and 2019 were collected from the patients’ histopathological records. Of the 120,010 oral and maxillofacial biopsies (2010-2019), 6.9% (8,321 cases) were diagnosed as lip (0.8%, 951 cases), oral cavity (4.9%, 5,971 cases), and oropharyngeal (1.2%, 1,399 cases) SCCs. Most cases were from Brazil’s Southeast (64.5%), where six of the OMPLs analyzed are located. The predominant profile of patients with lip and oral cavity SCC was Caucasian men, with a mean age over 60 years, low schooling level, and a previous history of heavy tobacco consumption. In the oropharyngeal group, the majority were non-Caucasian men, with a mean age under 60 years, had a low education level, and were former/current tobacco and alcohol users. According to data from the Brazilian National Cancer Institute, approximately 9.9% of the total lip, oral cavity, and oropharyngeal SCCs reported over the last decade in Brazil may have been diagnosed at the OMPLs included in the current study. Therefore, this data confirms the contribution of public OMPLs with respect to the important diagnostic support they provide to the oral healthcare services extended by the Brazilian Public Health System.

https://doi.org/10.1590/1807-3107bor-2023.vol37.0126

Obatoclax reduces cell viability of acute myeloid leukemia cell lines independently of their sensitivity to venetoclax
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Lima, Keli

; Vicari, Hugo Passos

; Carlos, Jorge Antonio Elias Godoy

; da Silva, Jean Carlos Lipreri

; Figueiredo-Pontes, Lorena Lobo de

; Rego, Eduardo Magalhães

; Machado-Neto, João Agostinho

Hematology, Transfusion and Cell Therapy

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Jan 2022, Volume 44 Nº 1 Páginas 124 - 127

Resumo: En | Texto: En | PDF: En

https://doi.org/10.1016/j.htct.2021.01.004

Structure, Biomass and Diversity of a Late-Successional Subtropical Atlantic Forest in Brazil
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Oliveira, Laio Zimermann

; Maçaneiro, João Paulo de

; Silva, Daniel Augusto da

; Uller, Heitor Felippe

; Britto, Pedro Caldas de

; Correia, Jean

; Piazza, Geferson Elias

; Zambiazi, Daisy Christiane

; Vibrans, Alexander Christian

; Fantini, Alfredo Celso

Floresta e Ambiente

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2022, Volume 29 Nº 4 elocation e20210095

Resumo: En | Texto: En | PDF: En

Abstract The highly biodiverse Atlantic Forest has been heavily exploited and its remnant areas, which are mostly composed of secondary forests, cover less than 20% of its original extension. This study analyzed the structure, aboveground biomass (AGB) and species diversity of a secondary evergreen rainforest in the state of Santa Catarina, southern Brazil. Individuals with diameter at breast height ≥ 5 cm on 22 sample plots were measured, totalizing 3.49 hectares of sampled area. A mean AGB of 201.6 Mg ha-1 (standard deviation = 35.5) was found; Hyeronima alchorneoides and Miconia cinnamomifolia represented 29.1% and 7.6% of the community AGB ha-1, respectively. A total of 153 species distributed in 107 genera and 55 families was observed; it was estimated that 121 species (95% confidence interval: [117, 125]) occurred in one hectare. These results highlight the importance of secondary forests for biodiversity restoration/conservation and carbon sequestration.

https://doi.org/10.1590/2179-8087-floram-2021-0095

Geostatistical-based enhancement of RFEM regarding reproduction of spatial correlation structures and conditional simulations
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Belo, Jean Lucas dos Passos

; Queiroz, Paulo Ivo Braga de

; Silva, Jefferson Lins da

Soils and Rocks

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2022, Volume 45 Nº 4 elocation e2022076121

Resumo: En | Texto: En | PDF: En

Abstract Engineering always deals with uncertainties, and efforts are needed to quantify them. A probabilistic analysis considers the statistical information of the problem to this quantification. In the geotechnical area, uncertainties play a particular role in structure design because it deals with naturally formed materials. Evaluating spatial variability has become progressively important. However, studies on the correct reproduction of this variability and conditional simulations are limited. In this paper, a geostatistical-based enhancement of the Random Finite Element Method (RFEM) is presented. The main aim of this study is to incorporate an advanced multivariate geostatistical technique (i.e., Turning Bands Co-simulation, TBCOSIM) to reproduce the coregionalization model of soil properties correctly in order to investigate the effects regarding this reproduction. It is illustrated in a real case of soil slope. The results showed that, for the unconditional simulation, the presented approach reached a perfect agreement with the coregionalization model, while the conditional simulation inserted some disturbances to this agreement, but it still satisfactorily reproduced the model. The original RFEM failed to reproduce this structure, leading to lower variances than the presented approach, which would cause a non-conservative design. Furthermore, disregarding the local uncertainty (i.e., the nugget effect) may introduce bias to analysis and, depending on its magnitude, may also lead the conditional analysis to not show a worthwhile reduction in variances of results. Finally, this paper shows that correctly determining the coregionalization model and reproducing it on probabilistic analysis may meaningfully influence the results.

https://doi.org/10.28927/sr.2022.076121

10.

Use of hyaluronic acid associated with triamcinolone acetonide or ozone gas in the treatment of induced osteoarthritis in rabbits
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Brombini, Giovanna Cristina

; Rahal, Sheila Canevese

; Fabro, Alexandre Todorovic

; Santos, Ivan Felismino Charas dos

; Tsunemi, Miriam Harumi

; Joaquim, Jean Guilherme Fernandes

; Doiche, Danuta Pulz

; Silva, Jeana Pereira da

; Mamprim, Maria Jaqueline

Acta Cirúrgica Brasileira

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2021, Volume 36 Nº 12 elocation e361201

Resumo: En | Texto: En | PDF: En

ABSTRACT Purpose: To evaluate the effects of the intra-articular application of hyaluronic acid associated with triamcinolone acetonide, and ozone gas in the treatment of induced osteoarthritis in rabbit’s stifles. Methods: Twenty-one Norfolk rabbits were submitted to cranial cruciate ligament transection of the left stifle. After six weeks of the surgery, the rabbits were randomized assigned into three groups: G1 (control) – saline solution (0.9%); G2 – hyaluronic acid associated with triamcinolone; G3 – ozone gas, submitted to three intra-articular applications every seven days. Results: Significant differences occurred: osteophytes at medial femoral condyle (G2 > G1, G2 > G3) on radiography exam; thickening of the medial condyle (G1 > G3, G2 > G3) on ultrasound exam; osteophytes at lateral tibial condyle (G2 > G1, G2 > G3), and medial femoral condyle (G1 > G2, G3 > G1) on computed tomography. Histologically, mean values of chondrocytes in the femur and tibia in G3 and G2 were statistically lower. Conclusions: The intra-articular injection of hyaluronic acid associated with triamcinolone accentuated degenerative joint disease by imaging and macroscopic evaluations, and by histological findings, this treatment and the ozone gas treatment showed similar effects and were inferior to the saline solution (0.9%).

https://doi.org/10.1590/acb361201 2 downloads

11.

COVID-19: The question of genetic diversity and therapeutic intervention approaches
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Figueiredo, David Livingstone Alves

; Ximenez, João Paulo Bianchi

; Seiva, Fábio Rodrigues Ferreira

; Panis, Carolina

; Bezerra, Rafael dos Santos

; Ferrasa, Adriano

; Cecchini, Alessandra Lourenço

; Medeiros, Alexandra Ivo de

; Almeida, Ana Marisa Fusco

; Ramão, Anelisa

; Boldt, Angelica Beate Winter

; Moya, Carla Fredrichsen

; Chin, Chung Man

; Paula, Daniel de

; Rech, Daniel

; Gradia, Daniela Fiori

; Malheiros, Danielle

; Venturini, Danielle

; Tavares, Eliandro Reis

; Carraro, Emerson

; Ribeiro, Enilze Maria de Souza Fonseca

; Pereira, Evani Marques

; Tuon, Felipe Francisco

; Follador, Franciele Aní Caovilla

; Fernandes, Glaura Scantamburlo Alves

; Volpato, Hélito

; Cólus, Ilce Mara de Syllos

; Oliveira, Jaqueline Carvalho de

; Rodrigues, Jean Henrique da Silva

; Santos, Jean Leandro dos

; Visentainer, Jeane Eliete Laguila

; Brandi, Juliana Cristina

; Serpeloni, Juliana Mara

; Bonini, Juliana Sartori

; Oliveira, Karen Brajão de

; Fiorentin, Karine

; Lucio, Léia Carolina

; Faccin-Galhardi, Ligia Carla

; Ferreto, Lirane Elize Defante

; Lioni, Lucy Megumi Yamauchi

; Consolaro, Marcia Edilaine Lopes

; Vicari, Marcelo Ricardo

; Arbex, Marcos Abdo

; Pileggi, Marcos

; Watanabe, Maria Angelica Ehara

; Costa, Maria Antônia Ramos

; Giannini, Maria José S. Mendes

; Amarante, Marla Karine

; Khalil, Najeh Maissar

; Lima Neto, Quirino Alves de

; Herai, Roberto H.

; Guembarovski, Roberta Losi

; Shinsato, Rogério N.

; Mainardes, Rubiana Mara

; Giuliatti, Silvana

; Yamada-Ogatta, Sueli Fumie

; Gerber, Viviane Knuppel de Quadros

; Pavanelli, Wander Rogério

; Silva, Weber Claudio da

; Petzl-Erler, Maria Luiza

; Valente, Valeria

; Soares, Christiane Pienna

; Cavalli, Luciane Regina

; Silva Jr, Wilson Araujo

Genetics and Molecular Biology

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2021, Volume 44 Nº 1 elocation e20200452

Resumo: En | Texto: En | PDF: En

Abstract Coronavirus disease 2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome Coronavirus type 2 (SARS-CoV-2), is the largest pandemic in modern history with very high infection rates and considerable mortality. The disease, which emerged in China’s Wuhan province, had its first reported case on December 29, 2019, and spread rapidly worldwide. On March 11, 2020, the World Health Organization (WHO) declared the COVID-19 outbreak a pandemic and global health emergency. Since the outbreak, efforts to develop COVID-19 vaccines, engineer new drugs, and evaluate existing ones for drug repurposing have been intensively undertaken to find ways to control this pandemic. COVID-19 therapeutic strategies aim to impair molecular pathways involved in the virus entrance and replication or interfere in the patients’ overreaction and immunopathology. Moreover, nanotechnology could be an approach to boost the activity of new drugs. Several COVID-19 vaccine candidates have received emergency-use or full authorization in one or more countries, and others are being developed and tested. This review assesses the different strategies currently proposed to control COVID-19 and the issues or limitations imposed on some approaches by the human and viral genetic variability.

https://doi.org/10.1590/1678-4685-gmb-2020-0452

12.

Morte nos Cuidados Paliativos: Representações Sociais de uma Equipe Multidisciplinar
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Fitaroni, Juliana Batista

; Bousfield, Andréa Barbará da Silva

; Silva, Jean Paulo da

Psicologia: Ciência e Profissão

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2021, Volume 41 elocation e209676

Resumo: Pt En Es | Texto: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Resumo O objetivo deste estudo é apreender tanto as representações sociais de morte como a compreensão desse saber, construído na prática profissional por uma equipe multidisciplinar de cuidados paliativos. Participaram da investigação 20 profissionais de uma equipe multidisciplinar de cuidados paliativos que trabalhavam há pelo menos seis meses na equipe que aceitou participar da pesquisa. Trata-se de um estudo de abordagem qualitativa com delineamento de estudo de casos. A coleta de dados foi realizada por meio de entrevista individual em profundidade. A avaliação dos dados empregou a análise de conteúdo do tipo categorial, com o suporte do software Atlas.ti. Como resultado, afirma-se que os participantes compreendem a morte tanto como parte do processo de vida, quanto como um evento capaz de gerar perdas. Encontraram-se evidências de que a representação social de morte está em transformação nesse grupo, pois as trocas e compartilhamentos de experiências, pautados na filosofia paliativista, fazem com que os profissionais questionem sua própria forma de encarar a morte.

Abstract This study aims at comprehending the social representations of death and the understanding of this knowledge built in professional practice experienced by a multidisciplinary team of palliative care. In total, 20 professionals of a multidisciplinary team of Palliative Care participated in this study, who worked there for at least 6 months in the team and who agreed to participate in the study. This is a qualitative approach research with case study design. The data collection was conducted using individual in-depth interviews. The data were analyzed using the content analysis of categorical type, with the support of the software Atlas.ti. The participants understand death as part of the life process, but which generates losses. Evidences were found that the social representation of death is under transformation, because the exchanging and sharing of experiences with the members of the multidisciplinary team, guided by the palliative philosophy, make the professionals to question themselves about their way of facing death.

Resumen El objetivo de este estudio fue comprender las representaciones sociales de la muerte y la comprensión de este conocimiento en la práctica profesional de un equipo multidisciplinario de cuidados paliativos. Participaron en la investigación 20 profesionales de un equipo multidisciplinario de cuidados paliativos que trabajaban hace al menos seis meses en el equipo. Este es un estudio cualitativo con delineamiento de estudio de casos. La recopilación de datos se realizó mediante la utilización de entrevista individual en profundidad. Para el análisis de datos se empleó el análisis de contenido del tipo categorial, con el soporte del software Atlas.ti. Los resultados evidencian que los participantes comprenden la muerte como parte del proceso de la vida, que puede generar pérdidas. Se encontraron que la representación social de la muerte está en transformación para ese grupo, pues los intercambios y experiencias compartidas, pautados en la filosofía paliativista, hacen que los profesionales cuestionen su forma de encarar la muerte.

https://doi.org/10.1590/1982-3703003209676 399 downloads

13.

Influence of early use of antimicrobial on the health and performance of Holstein calves in the first month of life
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Martin, Camila C.

; Basqueira, Natália S.

; Ramos, Jean S.

; Silva, Karen N.

; Baccili, Camila C.

; Brandão, Paulo E.

; Gomes, Viviani

Pesquisa Veterinária Brasileira

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Jan 2020, Volume 40 Nº 1 Páginas 17 - 28

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: O uso precoce de antimicrobianos tem sido adotado em muitas fazendas para profilaxia das diarreias e doença respiratória em bezerras, no entanto existem controvérsias sobre os beneficios desta prática na saúde desses animais. Esta pesquisa avaliou a influência do uso precoce de antimicrobiano na sanidade e desempenho de bezerras holandesas recém-nascidas. Para tanto foram selecionadas 26 bezerras Holandesas distribuídas de acordo com a aplicação (ATB+) ou não (ATB-) de tulatromicina (2,5mg/Kg) por via subcutânea até 12h de vida. As bezerras foram examinadas por meio de exame clínico geral, escore fecal, escore respiratório e palpação externa da região umbilical, além da matéria seca fecal. A presença de anemias foi determinada pelo eritrograma utilizando sistema automático e além da dosagem de ferro utilizando kit comercial. O diagnóstico etiológico das diarreias foi investigado por meio da técnica de flutuação em solução saturada de sacarose (Cryptosporidium) e multiplex semi-nested RT-PCR (rotavírus/coronavírus). O desempenho das bezerras foi estimado pelo ganho de peso. As bezerras foram avaliadas até doze horas após o nascimento (≤12h); 3-5º (D3-5); 7-9º (D7-9); 13-15º (D13-15); 20-23º (D20-23); e 27-30º dias de vida (D27-30). Não foram encontradas diferenças entre os grupos ATB+ e ATB- em relação à frequência cardíaca, frequência respiratória e temperatura retal. O eritrograma revelou maior frequência de anemias no grupo ATB- (P=0,016) no D3-5. Neste momento também foram observados menores valores de ferro sérico (P=0,051). Foram detectados treze casos de doença respiratória durante o estudo, no entanto não foi possível detectar diferença entre os grupos. A frequência de diarreias (escore fecal 2 e 3) foi alta em ambos os grupos, observando-se pico no D13-15 (ATB+=92,3%; ATB-=92,3%). Não observamos diferenças entre os grupos em relação a frequência de diarreia considerando-se a matéria seca fecal. O agente etiológico predominante nas diarreias foi o Cryptosporidium. O ganho de peso diário foi igual entre grupos, com intensa redução no GPD no D13-15. A administração de tulatromicina na dose profilática (2,5mg/Kg) ao nascimento diminuiu a frequência de anemias e não influenciou no ganho de peso e prevalência de diarreias.

ABSTRACT: The early use of antimicrobial therapy has been introduced in many farms to prevent diarrhea and respiratory disease in young calves; however, there is controversy about whether this practice has a beneficial effect on the health of these animals. This study evaluated the influence of the early use of antimicrobials on the health and performance of neonatal Holstein calves. Twenty-six Holstein calves were screened and divided into two groups, according to the administration (ATB+), or not (ATB-) of tulathromycin (2.5mg/kg, subcutaneously) within the first 12 hours of life. Calves were evaluated by general clinical examination, fecal score, respiratory score, and external palpation of the umbilical region, besides fecal output of dry matter. Anemia was determined by using an automatic system and, also, using a commercial kit for iron dosage. Diarrhea was diagnosed by a centrifuge-flotation technique using a sugar solution (Cryptosporidium) and multiplex semi-nested RT-PCR (rotavirus/coronavirus). The performance of the calves was estimated by Daily Weight Gain (DWG). The young dairy calves were evaluated within 12 hours of birth (≤12h) and at 3-5th (D3-5), 7-9th (D7-9), 13-15th (D13-15), 20-23rd (D20-23), and 27-30th (D27-30) days of life. No difference was noted between the ATB+ and ATB- groups concerning heart rate, respiratory frequency, and rectal temperature. Erythrogram showed a higher frequency of anemia in ATB- group (P=0.016) at the D3-5 check-up; lower values of serum iron were also observed simultaneously (P=0.051). Thirteen cases of respiratory disease were detected during this study; however, no significant difference was observed between the groups in this regard. The frequency of diarrhea (fecal score 2-3) was high in both groups, peaking at D13-D15. No differences were noted between the groups regarding the frequency of diarrhea when considering the dry fecal matter. The predominant etiological agent for diarrhea was Cryptosporidium spp.. The DWG was similar between groups, with maximum weight reduction on D13-15. The administration of tulathromycin in prophylactic dose (2.5mg/kg) at birth decreased the frequency of anemia but did not influence weight gain or the prevalence of diarrhea.

https://doi.org/10.1590/1678-5150-pvb-5722 1209 downloads

14.

Physical fractions of organic matter and mineralizable soil carbon in forest fragments of the Atlantic Forest
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Ozório, Jefferson Matheus Barros

; Rosset, Jean Sérgio

; Schiavo, Jolimar Antonio

; Souza, Camila Beatriz da Silva

; Farias, Paulo Guilherme da Silva

; Oliveira, Naelmo de Souza

; Menezes, Roniedison da Silva

; Panachuki, Eloi

Revista Ambiente & Água

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2020, Volume 15 Nº 6 elocation e2601

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo O objetivo do presente trabalho foi determinar o fracionamento físico-granulométrico e avaliar o carbono mineralizável no interior e ao entorno de fragmentos florestais do bioma Mata Atlântica localizados no estado do Paraná. As amostras de solo foram coletas em três de três pontos internos dos fragmentos: borda (BO), metade do raio (MR) e centro (CF) e um ponto em áreas de sistema plantio direto (SPD) no entorno dos fragmentos, em quatro repetições. Foram determinados os teores de carbono orgânico total (COT), carbono da fração particulada (C-MOP) e da fração mineral (C-MOM), sendo calculados as %MOP e %MOM e os estoques de MOP (Est. MOP) e de MOM (Est. MOM), além dos índices: índice de estoque de carbono (IEC), labilidade (L), índice de labilidade (IL) e índice de manejo de carbono (IMC), avaliando ainda a emissão de CO2, diária e acumulada. Os maiores teores de COT foram observados no ponto CF. Os maiores teores de C-MOP foram observados nos pontos BO e CF do fragmento 1, no ponto CF do fragmento 2, e os maiores teores de C-MOM foram expressos no ponto CF de ambos os fragmentos. O IMC apresentou padrão distinto entre os fragmentos. As áreas de SPD apresentaram menor emissão de C-CO2, com 39,8% e 28,3% menos emissão total em relação ao CF. Os resultados do fracionamento físico-granulométrico mostram o ponto CF favorece a qualidade da MOS e a análise de carbono mineralizável indicou que a conversão de áreas nativas em SPD compromete a atividade microbiana do solo.

Abstract This study determined the physical granulometric fractionation evaluated the mineralizable carbon within and around forest fragments of the Atlantic Forest biome located in the state of Paraná. Soil samples were collected at three three internal points of the fragments: the edge (E), the half radius (HR) and the center (CF); and one point in no-tillage system (NTS) areas around the fragments, in four replicates. The contents of total organic carbon (TOC), particulate fraction carbon (C-POM) and mineral fraction (C-MOM) were determined, and the %POM and %MOM and the stocks of POM (StockPOM) and MOM (StockMOM) were calculated, in addition to the indices: carbon stock index (CSI), lability (L), lability index (LI) and carbon management index (CMI), also evaluating CO2 emission, daily and accumulated. The highest TOC levels were observed in the CF point. The highest C-POM contents were observed in the E and CF points of fragment 1, in the CF point of fragment 2, and the highest C-MOM contents were expressed in the CF points of both fragments. CMI showed a distinct pattern among the fragments. The NTS areas showed lower C-CO2 emissions, with 39.8% and 28.3% less total emission compared to CF. The results of physical granulometric fractionation show the CF point favors the quality of SOM and the mineralizable carbon analysis indicated that the conversion of native areas into NTS compromises soil microbial activity.

https://doi.org/10.4136/ambi-agua.2601 472 downloads

15.

Effect of the type of milking on mammary gland examination in Saanen goats
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Ramos, Jean Silva

; Gomes, Viviani

; Matazo, Mariana Porto

; Silva, Cynthia Pereira da Costa e

; Baldacim, Vinicius Alvim Passos

; Madureira, Karina Medici

Arquivos do Instituto Biológico

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2020, Volume 87 elocation e0802018

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Esta pesquisa avaliou o efeito da ordenha sobre o exame físico da glândula mamária (GM) e do leite em cabras Saanen. Seis propriedades foram selecionadas, quatro fazendas foram utilizadas após avaliação da seleção dos critérios e distribuídas em dois grupos de acordo com o método de ordenha: manual ou mecânico. O exame físico da GM foi realizado utilizando as técnicas de inspeção e palpação, além do exame do leite pelo teste da caneca de fundo escuro, California Mastitis Test, contagem de células somáticas e exame bacteriológico. Foi possível detectar uma associação entre o método de ordenha com a maioria dos exames físicos da GM: inspeção da GM (p = 0,001), inspeção do teto (p = 0,002), palpação da GM (p = 0,054) e palpação do teto (p = 0,036). A ordenha mecânica associou-se com a redução do volume de GM (OR = 6,00), redução do tamanho do teto (OR = 16,19) e consistência firme do parênquima mamário (OR = 2,39). O uso da ordenha mecânica representou menor risco para o aumento do volume do volume da GM (OR = 0,288) e presença da cisternite (OR = 0,490). Também foi detectada a associação entre a CCS e o tipo de ordenha (p = 0,002). A ordenha mecânica foi associada com as alterações no exame físico de GM e do leite compatíveis com lesões traumáticas. Essas informações indicam o uso inadequado do equipamento de ordenha na criação de cabras leiteiras.

ABSTRACT This research evaluated the effect of milking on the physical exam of the mammary gland (MG) and milk examination in Saanen goats. Six properties were selected, four farms were used after evaluation of criteria selection and distributed in two groups according to the milking method: manual or mechanical. The physical examination of MG was performed using the techniques of inspection and palpation, in addition to the examination of the milk by strip cup, California Mastitis Test, somatic cell count, and bacteriological exam. It was possible to detect an association between the milking method with the most of physical exam of the MG: inspection of the MG (p = 0.001), inspection of the teat (p = 0.002), palpation of the MG (p = 0.054) and palpation of the teat (p = 0.036). The machine milking had an association with the reduction of the volume of MG (OR = 6.00), reduction of the teat size (OR = 16.19), and firm consistency of mammary parenchyma (OR = 2.39). The use of machine milking represented a less risk for an increase of the volume of the MG volume (OR = 0.288) and the presence of cisternitis (OR = 0.490). The association between the SCC and the type of milking was also detected (p = 0.002). Machine milking was associated with changesin the physical exam of MG and milk examination compatible with traumatic lesions. This information indicates inappropriate use of milking equipment in the dairy goat’s properties.

https://doi.org/10.1590/1808-1657000802018 671 downloads

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